Info For Group Members
Recording of DQF-COSY-spectra on the Bruker 500-2
(Please refer also to the Bruker manual)
- Determine T1
- rpar DQFCOSY.BBI
- eda: set SW, O1P to the smallest window
possible. Use the same values for F1 and F2. Set AQ (.5-2 s) as
desired.
- set P1 as determined above and D1 to 1.25xT1, RG
to the rg-value in the 1D experiment + 10. Set eda-TD to
1k in F2 and 256 in F1.
- choose NS in multiples of 8
- check the required experiment time with expt. If the time
is to long, either reduce NS or the number of experiments in F1
(eda - TD(F1)=128-512)
- Start experiment: ZG. The spectrum accumulated so far can
be viewed any time by xfb.
Phasing:
- xfb (can be entered anytime during spectrum acquisition to
check the spectrum achieved so far, but MUST be entered at the end of
data acquisition. Each experiment increases the resolution in F1.
Comparable to tr in 1D.)
- The standard setting is to display a magnitude spectrum and
phasing is neither required nor possible. To display a phase sensitive
spectrum, type edp and change phase correction from mc
to pk for F2 and F1.
- Hit the phase button and proceed as for other 2D spectrum. Note:
Diagonal peaks in DQF-COSY have ALWAYS a dispersion part and can never
be phased correctly. Correct phasing of the cross peaks is possible
(though difficult). Please refer to the Bruker manual on DQF-COSY.
Plotting:
- rpar standard2d: Load only the plot parameters (clicking
on plot)
- edg: Adjust the values as needed, refer also to the bruker
manual.
- Zoom the desired region, click on Def-Plot, plot.
