Info For Group Members

NOESY-spectra on the Bruker 500-2

(Please refer also to the Bruker manual)

Determine T1
rpar NOESYTP.BBI
eda: set SW, O1P to the smallest window possible. Use the same values for F1 and F2. Set AQ (.5-2 s) as desired.
set P1 as determined above and D1 to 1.25xT1, RG to the rg-value in the 1D experiment + 10, and D8 to approximately T1.
choose NS in multiples of 8
check the required experiment time with expt. If the time is to long, either reduce NS or the number of experiments in F1 (eda - TD(F1)=128-512)
Start experiment: ZG. The parameter to adjust to achieve better crosspeaks is D8.

Phasing:

xfb (can be entered anytime during spectrum acquisition to check the spectrum achieved so far, but MUST be entered at the end of data acquisition. Each experiment increases the resolution in F1. Comparable to tr in 1D.)
rser 1 (reads the first row/first spectrum in F1) - sinm (window function for 2D-experiments) - ft (NO ef) - phase the spectrum - return - save as 2D - click 2D-button to return to 2D spectrum
Phasing of F1 and fine-adjustement F2-phasing can be made by hitting the phase-button (nicely explained in the Bruker manual)
Once phasing is done, set the phase correction in edp to pk in F1 and F2 to adjust the phases automatically every time you enter xfb.

Improving the spectrum:

edp: Play around with different settings for BC in F1. Do xfb after saving the parameters.
abs1, abs2 performs baseline correction in F1 and F2. Please check that you don't introduce artificial peaks!
sym symmetrizes the spectra and eliminates T1-noise. ATTENTION: sym will ALWAYS generate positive crosspeaks, since all peaks show T1-noise! Small negative crosspeaks might thus be eliminated. Improving digital resolution:
edp: Double the SI-parameter for F1 and F2

Plotting: